Integrated microfluidic device for the separation and electrochemical detection of catechol estrogen-derived DNA adducts

Catechol estrogen-derived DNA adducts are formed as a result of the reaction of catechol estrogen metabolites (e.g., catechol estrogen quinones) with DNA to form depurinating adducts. Developing a new methodology for the detection of various DNA adducts is essential for medical diagnostics, and to this end, we demonstrate the applicability of on-chip capillary electrophoresis with an integrated electrochemical system for the separation and amperometric detection of various catechol estrogen-derived DNA adducts. A hybrid PDMS/glass microchip with in-channel amperometric detection interfaced with in situ palladium decoupler is utilized and presented. The influence of buffer additives along with the effect of the separation voltage on the resolving power of the microchip is discussed. Calibration plots were constructed in the range 0.4–10 μM with r ² ≥ 0.999, and detection limits in the attomole range are reported. These results suggest that on-chip analysis is applicable for analyzing various DNA adducts as potential biomarkers for future medical diagnostics.     

Light driven reactions of single physisorbed azobenzenes

We present a successful attempt of decoupling a dye molecule from a metallic surface via physisorption for enabling direct photoisomerization. Effective switching between the isomers is possible by exposure to UV light via the rotation pathway.     

Retrieving the heat transfer coefficient for jet impingement from transient temperature measurements

Algorithm of retrieving the heat transfer coefficient (HTC) from transient temperature measurements is presented. The unknown distributions of two types of boundary conditions: the temperature and heat flux are parameterized using a small number of user defined functions. The solutions of the direct heat conduction problems with known boundary temperature and flux are expressed as a superposition of auxiliary temperature fields multiplied by unknown parameters. Inverse problem is formulated as a least squares fit of calculated and measured temperatures and is cast in a form of a sum of two objective functions. The first results originates from an inverse problem for retrieving the boundary temperature the second comes from the inverse problem for reproducing the boundary heat flux. The final form of the objective function is obtained by enforcing constant in time value of the heat transfer coefficient. This approach leads to substantial regularization of the results, when compared with the standard technique, where HTC is calculated from separately reconstructed temperature and heat flux on the boundary. The validation of the numerical procedure is carried out by reconstructing a known distribution of the HTC using simulated measurements laden by stochastic error. The proposed approach is also used to reconstruct the distribution of the HTC in a physical experiment of heating a cylindrical sample using an impinging jet.     

Deformations of algebroid stacks

In this paper we consider deformations of an algebroid stack on an étale groupoid. We construct a differential graded Lie algebra (DGLA) which controls this deformation theory. In the case when the algebroid is a twisted form of functions we show that this DGLA is quasiisomorphic to the twist of the DGLA of Hochschild cochains on the algebra of functions on the groupoid by the characteristic class of the corresponding gerbe.     

The Baum-Connes conjecture via localisation of categories

We redefine the Baum-Connes assembly map using simplicial approximation in the equivariant Kasparov category. This new interpretation is ideal for studying functorial properties and gives analogues of the Baum-Connes assembly map for other equivariant homology theories. We extend many of the known techniques for proving the Baum-Connes conjecture to this more general setting.     

Algebraic index theorem

We prove the Atiyah-Singer index theorem where the algebra of pseudodifferential operators is replaced by an arbitrary deformation quantization of the algebra of functions on a symplectic manifold.Partially supported by NSF Grant DMS-9101817.     

Electron impact-induced fragmentation of some aminonitropyridines

The fragmentation behaviour of 18 vicinally substituted aminonitropyridines was studied under electron impact conditions. Tbe decomposition patterns were found to be strongly dependent on the position of substituents. The formation of the aza analogue of carbazole seems to affect considerably the fragmentation of 3-nitro-2-phenylaminopyridine.     

Unitarization of Körner-Kuroda model of electromagnetic structure of octet 1/2+ baryons

The Körner-Kuroda model of the electromagnetic structure of octet 1/2⁺ baryons is restored on a more topical physical basis. Electromagnetic radii of baryons under consideration are calculated and compared with other model predictions. By an incorporation of a two-cut approximation of correct form factor analytic properties and nonzero vector-meson widths, the Körner-Kuroda model is unitarized, providing in this manner imaginary parts of the octet 1/2⁺ baryon form factors to be nonzero just starting from a branch point corresponding to the lowest threshold.     

The magnetic field,force and inductance of the system consisting of a thin ellipsoidal coil and a ferromagnetic ellipsoid

The method of separation of variables has been applied to determine the magnetic field of the system consisting of a thin ellipsoidal coil and a ferromagnetic ellipsoid of revolution. The inductance and the force acting between the coil and the ellipsoid are also examined.